Cell Lines and Cell Cultures
Genscript Corporation CHO-K1/BDCA2 Stable Cell Line
BDCA-2 is a novel type II C-type lectin which shows 50 7% sequence identity at the amino acid level to its putative murine ortholog the murine dendritic cell associated C-type lectin 2 In addition to its antigen capturing function BDCA-2 can mediate potent inhibition of induction of IFN- / expression in PDCs Production of IFN- / in response to several different types of viruses bacteria CpG-DNA dsRNA and SLE serum is by far the most prominent feature of PDCs
Miltenyi Biotec, Inc. REAlease CD56 MicroBead Kit, human
Positive isolation from PBMCs with releasable MicroBeads and antibody fragments
Miltenyi Biotec, Inc. Leukopak CD19 MicroBead Kit, human
Positive isolation of CD19+ cells directly from Leukopak
Miltenyi Biotec, Inc. LRSC CD19 MicroBead Kit, human
Positive isolation of CD19+ cells directly from LRSC
Miltenyi Biotec, Inc. MACSxpress LRSC Starting Kit
With MACSxpress Technology, untouched human leukocyte subsets can be isolated from whole blood, buffy coats, and LRSCs (buffy cones) without density gradient centrifugation. The MACSxpress Separator enables cell isolation from up to 30 mL of sample in a single step and can be used with 5, 15, or 50 mL tubes. The MACSxpress Starting Kits contain a MACSxpress Separator, a MACSmix™ Tube Rotator, and a MACSxpress Isolation Kit free of choice; starting up has never been easier. Kit containing MACS Equipment and Reagent for magnetic cell separation directly from LRSC using MACSxpress Beads
New England Biolabs, Inc. dam-/dcm- Competent E. coli – 20 x 0.05 ml/tube
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.
Miltenyi Biotec, Inc. Tumor-Asso. Fibroblast Isolation Kit, m
The Tumor-Associated Fibroblast Isolation Kit, mouse enables the isolation of tissue-derived, tumor-associated fibroblasts from dissociated mouse tumors
Miltenyi Biotec, Inc. Neonatal Cardiomyocyte Isolation Kit,rat
Untouched isolation of neonatal cardiomyocytes from rat hearts
Miltenyi Biotec, Inc. MACSxpress LRSC CD4 TC Iso Kit, h
Untouched isolation of CD4+ cells directly from LRSC
Miltenyi Biotec, Inc. MACSxpress LRSC Pan TC Iso Kit, h
Untouched isolation of pan T cells directly from LRSC
Genscript Corporation CHO-K1/ADRA2C/G15 Stable Cell
The adrenoceptors ADRA2C is expressed in the brain kidney aorta lung skeletal muscle heart and spleen ADRA2C knockout mice exhibited disrupts presynaptic inhibition of noradrenaline release at low stimulation frequencies
Genscript Corporation CHO-K1/cyno CD155 Stable Cell
CD155 commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5) is a type I transmembrane single-span glycoprotein and belongs to the nectins and nectin-like (Necl) subfamily CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV) an etiologic agent of the central nervous system disease poliomyelitis The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells CD155 may assist in an efficient humoral immune response generated within the intestinal immune system It s been shown that CD155 can be recognized and bind by DNAM-1 and CD96 which promotes the adhension migration and NK-cell killing Thus inducing cell-mediated tumor-specific immunity
Genscript Corporation CHO-K1/GPBAR1 Stable Cell Line
The gene GPBRA1 encodes G protein-coupled bile acid receptor Bile acid-binding induces its internalization activation of extracellular signal-regulated kinase and intracellular cAMP production
Genscript Corporation CHO-K1/Cyno VISTA Stable Cell
V-domain Ig suppressor of T cell activation (VISTA) is a potent negative regulator of T-cell function that is expressed on hematopoietic cells VISTA levels are heightened within the tumor microenvironment in which its blockade can enhance antitumor immune responses in mice In humans blockade of the related programmed cell death 1 (PD-1) pathway has shown great potential in clinical immunotherapy trials
Genscript Corporation CHO-K1/KIR Stable Cell Line
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and are subsets of T cells The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM) while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals The ligands for several KIR proteins are subsets of HLA class I molecules thus KIR proteins are thought to play an important role in regulation of the immune response